| The title characters of the series are a family of machine-enhanced human beings possessing unique powers after being augmented with bionic technology, much like The Six Million Dollar Man and The Bionic Woman. Each family member is given specific bionic powers, and thus they form a superhero team named Bionic Six. |
| Name | Character | Episodes |
|---|---|---|
| Frank Welker | Mechanic | 65 |
| John Stephenson | Bionic-1 | 65 |
| Brian Tochi | Karate-1 | 65 |
| Alan Oppenheimer | Prof. Amadeus Sharp | 65 |
| Neil Ross | F.L.U.F.F.I. | 65 |
| Jennifer Darling | Madame-O (voice) | 65 |
| Arthur Burghardt | Edward Tully (voice) | 65 |
| Hal Rayle | Eric Bennett 'Sport-1' (voice) | 65 |