| Name | Character | Episodes |
|---|---|---|
| Larry D. Mann | 77 | |
| Len Carlson | Green Goblin (voice) (uncredited) | 77 |
| Paul Soles | Peter Park / Spider-Man (voice) | 77 |
| Corinne Conley | 77 | |
| Peg Dixon | Mary Jane Watson / May Parker / Betty Brant (voice) | 77 |
| Paul Kligman | J. Jonah Jameson (voice) | 77 |
| Alfie Scopp | Artie (voice) (uncredited) | 57 |
| Chris Wiggins | Police officer / Director / Mysterio (voice) (uncredited) | 1 |
| Henry Ramer | Henry Smythe (voice) (uncredited) | 1 |
| Henry Ramer | Doctor Noah Boddy (voice) (uncredited) | 1 |