| The adventures of the elite G.I. Joe unit, whose members: Duke, Snake Eyes, Scarlett, Roadblock, Tunnel Rat and Ripcord were charged with crimes they did not commit. In order to clear themselves of the charges, they must expose the real culprits, who belong to the powerful corporation COBRA Industries. |
| Name | Character | Episodes |
|---|---|---|
| Kevin Michael Richardson | Roadblock (voice) | 26 |
| Johnny Messner | Flint (voice) | 26 |
| Natalia Cigliuti | Scarlett (voice) | 26 |
| Charlie Schlatter | Dr. Mindbender (voice) | 26 |
| Jason Marsden | Duke (voice) | 26 |
| Charlie Adler | Cobra Commander (voice) | 26 |
| Matthew Yang King | Tunnel Rat (voice) | 26 |
| Nika Futterman | Lady Jaye (voice) | 26 |
| Khary Payton | Ripcord (voice) | 7 |
| Clancy Brown | Destro (voice) | 4 |
| Andrew Kishino | Storm Shadow (voice) | 4 |
| Clancy Brown | James McCullen (voice) | 3 |
| Kim Mai Guest | Jinx (voice) | 3 |
| Lee Majors | General Abernathy (voice) | 2 |
| Greg Ellis | Breaker (voice) | 2 |
| Danny Cooksey | Young Snake Eyes (voice) | 2 |
| André Sogliuzzo | Major Bludd (voice) | 2 |
| Keone Young | 2 | |
| Peter MacNicol | Firefly (voice) | 1 |
| Jerry O'Connell | Barbecue (voice) | 1 |
| Jennifer Hale | Wendy (voice) | 1 |
| Fred Willard | Mayor Lockridge (voice) | 1 |
| James Arnold Taylor | Sherriff Matty / Russell Clemens (voice) | 1 |
| Brian Bloom | Zartan (voice) | 1 |
| Scott Menville | Vincent Hauser (voice) | 1 |
| Michael Bell | Max Hauser (voice) | 1 |
| Keith Szarabajka | 1 | |
| Jim Meskimen | 1 | |
| Stephen Stanton | 1 | |
| Travis Willingham | 1 | |
| Corey Burton | 1 |