| Name | Character | Episodes |
|---|---|---|
| Ayako Kawasumi | Saber (voice) | 25 |
| Akio Otsuka | Rider (voice) | 25 |
| Daisuke Namikawa | Waver Velvet (voice) | 25 |
| Rikiya Koyama | Kiritsugu Emiya (voice) | 25 |
| Tomokazu Seki | Archer (voice) | 25 |
| Ryotaro Okiayu | Berserker (voice) | 25 |
| Show Hayami | Tokiomi Tohsaka (voice) | 25 |
| Megumi Toyoguchi | Sola-Ui Nuada-Re Sophia-Ri (voice) | 25 |
| Sayaka Ohara | Irisviel von Einzbern (voice) | 25 |
| Akira Ishida | Ryuunosuke Uryuu (voice) | 25 |
| Hikaru Midorikawa | Lancer (voice) | 25 |
| Satoshi Tsuruoka | Caster (voice) | 25 |
| Asami Seto | Kotone (voice) | 1 |
| Nobunaga Shimazaki | Assassin (voice) | 1 |
| Akeno Watanabe | Natalia (voice) | 1 |
| Mai Kadowaki | Illyasviel von Einzbern (voice) | 1 |
| Masane Tsukayama | Zouken Matou (voice) | 1 |
| Tamio Ohki | Great king (voice) | 1 |
| Naomi Kusumi | Fionn mac Cumhail (voice) | 1 |