| Vanessa Helsing, the daughter of famous vampire hunter and Dracula nemesis Abraham Van Helsing is resurrected five years in the future to find out that vampires have taken over the world and that she possesses unique power over them. She is humanity’s last hope to lead an offensive to take back what has been lost. |
| Name | Character | Episodes |
|---|---|---|
| Kelly Overton | Vanessa Van Helsing | 65 |
| Rukiya Bernard | Doc | 65 |
| Aleks Paunovic | Julius | 65 |
| Jonathan Scarfe | Axel Miller | 60 |
| Vincent Gale | Flesh | 52 |
| Trezzo Mahoro | Mohamad | 40 |
| Paul Johansson | Dimitri | 39 |
| Christopher Heyerdahl | Sam | 27 |
| Jennifer Cheon Garcia | Ivory | 24 |
| Keeya King | Violet | 21 |
| Nicole Muñoz | Jack | 21 |
| Tricia Helfer | Dracula | 20 |
| Hilary Jardine | Susan | 15 |
| Neal McDonough | Hansen | 14 |
| Missy Peregrym | Scarlett Harker | 14 |
| David Cubitt | John | 13 |
| John Cassini | Max Maddox | 13 |
| Tim Guinee | Ted | 13 |
| Anna Galvin | Avery | 8 |
| Jesse Stanley | Bathory | 8 |
| Jesse Stanley | Oracle | 8 |
| Heather Doerksen | Michaela | 7 |
| John Reardon | Dr. Harrison (as Johnny Riordan) | 4 |
| Peter Bryant | Vice President Eli Newton | 4 |
| Luvia Petersen | Sgt. Weathers | 4 |
| Dakota Daulby | Willem | 4 |
| Rowland Pidlubny | Scab | 4 |
| Kim Coates | Count Dalibor | 3 |
| Richard Harmon | Max Borman | 3 |
| Aaron Douglas | Colonel Nicholson | 3 |
| Stephen Lobo | President Davis Park | 3 |
| Ali Liebert | Nina | 3 |
| Donald Sales | Jeremy | 3 |
| Holly Dignard | Jennifer | 3 |
| Jill Teed | President Archer | 3 |
| Tim Perez | Biggs | 3 |
| Dan Cade | Roberto | 3 |
| Hannah Cheramy | Dylan | 2 |
| Cassidy Nugent | Tabby | 2 |
| Reese Alexander | General Lannister | 2 |
| Tammy Gillis | Monica | 2 |
| Cory Gruter-Andrew | Troy Johnson | 2 |
| Julie Lynn-Mortensen | Lillian Van Helsing | 2 |
| Peter Kelamis | Pauly | 2 |
| Macie Juiles | Callie | 2 |
| Donny Lucas | Chad Johnson | 2 |
| Kheon Clarke | Special Op #1 | 2 |
| Steve Bacic | Emmett's Dad | 1 |
| John Reardon | Dr. Harrison | 1 |
| Christina Jastrzembska | Mama | 1 |
| Laura Mennell | Rebecca | 1 |
| Michael Eklund | Abraham Van Helsing | 1 |
| Michael Eklund | Jacob Van Helsing | 1 |
| Bradley Stryker | Matty | 1 |
| Jess McLeod | Shelley | 1 |
| Jeffrey Ballard | Aaron | 1 |
| Nelson Wong | Nguyen | 1 |
| Michael Benyaer | Stefan | 1 |
| Miranda Edwards | Coach | 1 |
| Christian Convery | Vampire Child #4A | 1 |
| Toby Levins | Bosworth | 1 |
| Paul Wight | Carnage | 1 |
| Sandy Robson | Daniel | 1 |
| Matreya Scarrwener | Brittney | 1 |
| Tony Alcantar | Officer Rudolph | 1 |
| Ron Selmour | Lukas | 1 |
| Jeremy Jones | Deputy #2 | 1 |
| Shawn Stewart | Loveland Guard | 1 |
| Nelson Leis | Leonard | 1 |
| Darcy Laurie | Hopper | 1 |
| Mark Sweatman | Combatant | 1 |
| Natalie Sharp | Shona | 1 |
| Kyle Strauts | Dominik | 1 |
| Kat Ruston | Zuma | 1 |
| Zenia Marshall | Kim | 1 |
| Trevor Carroll | Trafficker | 1 |
| Adrian Hough | Edward Hawkins | 1 |
| Sergio Osuna | Private Mendoza | 1 |
| Patricia Cullen | Valerie Seward | 1 |
| Tanya Champoux | Woman | 1 |
| Klarc Jerome Wilson | Special Op #3 | 1 |
| Jen Araki | Young Abby Van Helsing | 1 |
| Chris Ippolito | Vampire #1 | 1 |
| Michael Denis | Ted Seward | 1 |
| Rhonda Dent | Charlotte | 1 |
| Khamisa Wilsher | Lucretia | 1 |
| Christina Lewall | Claire | 1 |
| Sean Millington | Large Man | 1 |
| Rami Kahlon | Shivani | 1 |
| Christopher Gerard | Blak Tek Guard | 1 |
| Adrian Petriw | Colin / Daywalker #1 | 1 |