| Name | Character | Episodes |
|---|---|---|
| Lena Headey | Jeopardy Mouse (voice) | 100 |
| Ed Gaughan | Baron Greenback / Quark / Pandaminion (voice) | 100 |
| Morwenna Banks | Dawn / Weevil Queen (voice) | 100 |
| Stephen Fry | Colonel K (voice) | 100 |
| Kayvan Novak | Dr. Loo-cifer / Isambard King Kong Brunel / Birch Badboy (voice) | 100 |
| Alexander Armstrong | Danger Mouse (voice) | 100 |
| Kevin Eldon | Penfold (voice) | 100 |
| Shauna Macdonald | Prof. Squawkencluck (voice) | 100 |
| Rasmus Hardiker | Count Duckula / Ian (voice) | 100 |
| Marc Silk | Nero (voice) | 100 |
| Dave Lamb | Narrator / Stiletto (voice) | 100 |
| Richard Ayoade | The Snowman (voice) | 5 |
| John Oliver | Augustus P. Crumhorn IV (voice) | 4 |
| Ben Willbond | Prof. Ham Hands (voice) | 2 |
| Christopher Eccleston | J. Woolington Sham (voice) | 1 |
| Brian Blessed | Santa Claus (voice) | 1 |